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Chemistry Discussion (HPLC)

Relate your discussion to the basics of chromatographic theory. 1. HPLC – what does the name of the method actually mean? HPLC is short for High-Performance Liquid Chromatography. Why is it “high performance”? 2. What are the basics of chromatographic theory? 3. What is elution order? What is the experimental order of elution given the type of chromatography? 4. Define accuracy and precision. What is error analysis? What are possible errors when performing HPLC? What errors could affect the peak and the concentration of the unknown and standard curve? What is a standard curve? 5. Describe the terms A, B, C, and mobile phase flow rate in the van Deemter equation as applied to HPLC separation. 6. How does the solvent percentage’s effect the retention time? How does it affect the peak shapes (height and width)? 8. How does the flow rate change affect the retention times? 9. What is resolution, Rs? Why is it an important parameter in the chromatography theory? The equation used to calculate Rs is as following, 10. What is the capacity factor, k’? The equation used to calculate k’ as following k’ =tr-tmtm. Why is it an important parameter in the chromatography theory?