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The enzyme argininosuccinate synthetase catalyzes the formation of argininosucci

The enzyme argininosuccinate synthetase catalyzes the formation of argininosuccinate from citrulline and aspartate in an ATP dependent manner ( reaction shown below): Citrulline + ATP+L-Asp→ Argininosuccinate +AMP+PPi In a test tube, the enzyme-catalyzed reaction is readily reversible , yet in a cell the enzyme-catalyzed reaction is highly irreversible. Provide a detailed thermodynamic/ chemical explanation as to why this reaction is largely irreversible in a cell but not in a test tube.